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Issue 27, 2019
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A gene-targeted polymerase-mediated strategy to identify O6-methylguanine damage

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Abstract

Detecting DNA adducts in cancer genes is important for understanding cancer etiology. This study reports a strategy to identify the mutagenic DNA adduct O6-methylguanine in K-Ras. The strategy involves selective replication past a synthetic primer when placed opposite O6-methylguanine. Future work can apply this approach to other cancer-relevant genes.

Graphical abstract: A gene-targeted polymerase-mediated strategy to identify O6-methylguanine damage

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Supplementary files

Article information


Submitted
11 Jan 2019
Accepted
05 Mar 2019
First published
06 Mar 2019

This article is Open Access

Chem. Commun., 2019,55, 3895-3898
Article type
Communication

A gene-targeted polymerase-mediated strategy to identify O6-methylguanine damage

C. M. N. Aloisi, S. J. Sturla and H. L. Gahlon, Chem. Commun., 2019, 55, 3895
DOI: 10.1039/C9CC00278B

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