Rapid and sensitive colorimetric detection of pathogens based on silver–urease interactions

Abstract

Herein, we demonstrate a facile and economic approach for colorimetric detection of microbial pathogens in drinking water, employing silver–urease interactions. In the presence of harmful pathogens, receptor coated silver nanoparticles (AgNPs) preferentially bind to the bacterial surface and urease catalytically elevates the pH of the solution, which is sensed by a pH responsive chromogenic dye. The assay demarcates bacterial contamination levels up to 10² cells mL⁻¹ in a field-friendly method.