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Issue 33, 2019
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Rapid and sensitive colorimetric detection of pathogens based on silver–urease interactions

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Abstract

Herein, we demonstrate a facile and economic approach for colorimetric detection of microbial pathogens in drinking water, employing silver–urease interactions. In the presence of harmful pathogens, receptor coated silver nanoparticles (AgNPs) preferentially bind to the bacterial surface and urease catalytically elevates the pH of the solution, which is sensed by a pH responsive chromogenic dye. The assay demarcates bacterial contamination levels up to 102 cells mL−1 in a field-friendly method.

Graphical abstract: Rapid and sensitive colorimetric detection of pathogens based on silver–urease interactions

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Publication details

The article was received on 09 Jan 2019, accepted on 08 Mar 2019 and first published on 09 Mar 2019


Article type: Communication
DOI: 10.1039/C9CC00225A
Chem. Commun., 2019,55, 4765-4768

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    Rapid and sensitive colorimetric detection of pathogens based on silver–urease interactions

    P. Singh, S. Kakkar, Bharti, R. Kumar and V. Bhalla, Chem. Commun., 2019, 55, 4765
    DOI: 10.1039/C9CC00225A

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