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Chiral separation of D/L-arginine with whole cells through an engineered FhuA nanochannel

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Abstract

Downstream processing to obtain enantiopure compounds from a racemic mixture relies mainly on crystallization. Natural transporters can specifically translocate enantiomers through membranes. Here a β-barrel transmembrane protein FhuA is re-engineered into a chiral channel protein (FhuAF4) to resolve racemic mixtures of D-/L-arginine. The engineered FhuAF4 variant exhibits an enantioselectivity (E-value) of 1.92 and an enantiomeric excess percentage (ee%) of 23.91 at 52.39% conversion. OmniChange mutant libraries at the computationally identified “filter-regions” likely help to identify FhuA variants for enantiomeric separation of other compounds.

Graphical abstract: Chiral separation of d/l-arginine with whole cells through an engineered FhuA nanochannel

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Publication details

The article was received on 07 Jan 2019, accepted on 19 Mar 2019 and first published on 21 Mar 2019


Article type: Communication
DOI: 10.1039/C9CC00154A
Citation: Chem. Commun., 2019, Advance Article

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    Chiral separation of D/L-arginine with whole cells through an engineered FhuA nanochannel

    D. Anand, G. V. Dhoke, J. Gehrmann, T. M. Garakani, M. D. Davari, M. Bocola, L. Zhu and U. Schwaneberg, Chem. Commun., 2019, Advance Article , DOI: 10.1039/C9CC00154A

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