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Switching the regioselectivity of two nitrilases toward succinonitrile by mutating the active center pocket key residues through a semi-rational engineering

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Abstract

The 163Ala in bll6402NIT and 172Trp in blr3397NIT played a significant role in directing nitrilase regioselectivity. Amino acid mutation of two nitrilases at these two sites could alter the size of the active site pocket and further switch nitrilase regioselectivity toward succinonitrile, which lays the foundation for subsequent research on regioselective nitrilase towards several dinitriles.

Graphical abstract: Switching the regioselectivity of two nitrilases toward succinonitrile by mutating the active center pocket key residues through a semi-rational engineering

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Publication details

The article was received on 21 Dec 2018, accepted on 12 Feb 2019 and first published on 13 Feb 2019


Article type: Communication
DOI: 10.1039/C8CC10110H
Citation: Chem. Commun., 2019, Advance Article

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    Switching the regioselectivity of two nitrilases toward succinonitrile by mutating the active center pocket key residues through a semi-rational engineering

    Z. Chen, S. Jiang, H. Wang, L. Wang and D. Wei, Chem. Commun., 2019, Advance Article , DOI: 10.1039/C8CC10110H

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