DEAD-box RNA helicases Dbp2, Ded1 and Mss116 bind to G-quadruplex nucleic acids and destabilize G-quadruplex RNA

Jun Gao; Alicia K. Byrd; Boris L. Zybailov; John C. Marecki; Michael J. Guderyon; Andrea D. Edwards; Shubeena Chib; Kirk L. West; Zachary J. Waldrip; Samuel G. Mackintosh; Zhaofeng Gao; Andrea A. Putnam; Eckhard Jankowsky; Kevin D. Raney

doi:10.1039/C8CC10091H

DEAD-box RNA helicases Dbp2, Ded1 and Mss116 bind to G-quadruplex nucleic acids and destabilize G-quadruplex RNA†

Jun Gao,

^a Alicia K. Byrd,^a Boris L. Zybailov,^a John C. Marecki,^a Michael J. Guderyon,^a Andrea D. Edwards,^a Shubeena Chib,^a Kirk L. West,^a Zachary J. Waldrip,^a Samuel G. Mackintosh,^a Zhaofeng Gao,^b Andrea A. Putnam,^b Eckhard Jankowsky^b and Kevin D. Raney*^a

Author affiliations

* Corresponding authors

^a Department of Biochemistry and Molecular Biology, College of Medicine, University of Arkansas for Medical Sciences, 4301 West Markham Street (Slot 516), Little Rock, Arkansas 72205, USA
E-mail: RaneyKevinD@uams.edu
Fax: +1-501-686-8169
Tel: +1-501-686-5244

^b Center for RNA Science and Therapeutics, Department of Biochemistry, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, USA

Abstract

We identified 29 G-quadruplex binding proteins by affinity purification and quantitative LC-MS/MS. We demonstrated that the DEAD-box RNA helicases Dbp2, Ded1 and Mss116 preferentially bind to G-quadruplex nucleic acids in vitro and destabilize RNA quadruplexes, suggesting new potential roles for these helicases in disruption of quadruplex structures in RNA.

Chemical Communications

DEAD-box RNA helicases Dbp2, Ded1 and Mss116 bind to G-quadruplex nucleic acids and destabilize G-quadruplex RNA†

Abstract

Supplementary files

Article information

Download Citation

Permissions

DEAD-box RNA helicases Dbp2, Ded1 and Mss116 bind to G-quadruplex nucleic acids and destabilize G-quadruplex RNA

Social activity

Search articles by author

Spotlight

Advertisements