Jump to main content
Jump to site search


Single-color multiplexing by the integration of high-resolution melting pattern recognition with loop-mediated isothermal amplification

Author affiliations

Abstract

We develop a single-color multiplexing strategy by the integration of high-resolution melting pattern recognition with loop-mediated isothermal amplification (LAMP). This strategy can identify multiple amplicons with a small DNA melting temperature (Tm) difference (∼0.2 °C) without the involvement of either multicolor labels or parallelized multiplexing, and it can sensitively detect LAMP amplicons with the initial DNA concentrations ranging from 10 to 108 copies.

Graphical abstract: Single-color multiplexing by the integration of high-resolution melting pattern recognition with loop-mediated isothermal amplification

Back to tab navigation

Supplementary files

Publication details

The article was received on 08 Dec 2018, accepted on 30 Jan 2019 and first published on 30 Jan 2019


Article type: Communication
DOI: 10.1039/C8CC09741K
Citation: Chem. Commun., 2019, Advance Article

  •   Request permissions

    Single-color multiplexing by the integration of high-resolution melting pattern recognition with loop-mediated isothermal amplification

    J. Dong, Q. Xu, C. Li and C. Zhang, Chem. Commun., 2019, Advance Article , DOI: 10.1039/C8CC09741K

Search articles by author

Spotlight

Advertisements