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Issue 48, 2019
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Enhancement of exon skipping activity by reduction in the secondary structure content of LNA-based splice-switching oligonucleotides

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Abstract

PAGE and UV melting analysis revealed that longer LNA-based splice-switching oligonucleotides (SSOs) formed secondary structures by themselves, reducing their effective concentration. To avoid such secondary structure formation, we introduced 7-deaza-2′-deoxyguanosine or 2′-deoxyinosine into the SSOs. These modified SSOs, with fewer secondary structures, showed higher exon skipping activities.

Graphical abstract: Enhancement of exon skipping activity by reduction in the secondary structure content of LNA-based splice-switching oligonucleotides

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Publication details

The article was received on 04 Dec 2018, accepted on 14 May 2019 and first published on 15 May 2019


Article type: Communication
DOI: 10.1039/C8CC09648A
Chem. Commun., 2019,55, 6850-6853

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    Enhancement of exon skipping activity by reduction in the secondary structure content of LNA-based splice-switching oligonucleotides

    T. Shimo, K. Tachibana, Y. Kawawaki, Y. Watahiki, T. Ishigaki, Y. Nakatsuji, T. Hara, J. Kawakami and S. Obika, Chem. Commun., 2019, 55, 6850
    DOI: 10.1039/C8CC09648A

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