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On-chip biosynthesis of GM1 pentasaccharide-related complex glycans

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Abstract

A functional glycan chip combined with on-chip enzymatic glycosylation was developed to prepare complex glycan sources and to apply glycan-involved applications simultaneously. GM3 trisaccharide, GM2 tetrasaccharide, and GM1 pentasaccharide were successfully directly biosynthesized on lactose-immobilized surfaces through three consecutive glycosyltransferase reactions along with small amounts of enzymes and donors, without any additional processes. Biosynthesized GM1 pentasaccharide-related complex glycans were demonstrated to provide information on the substrate specificity of whole cholera toxin. Thus, the proposed on-chip glycan biosynthesis system can provide a new direction toward obtaining complex glycan sources and complex glycan-involved applications such as glycan–protein interaction analysis and glycan biomarker-based diagnosis.

Graphical abstract: On-chip biosynthesis of GM1 pentasaccharide-related complex glycans

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Publication details

The article was received on 10 Aug 2018, accepted on 27 Nov 2018 and first published on 27 Nov 2018


Article type: Communication
DOI: 10.1039/C8CC06526H
Citation: Chem. Commun., 2019, Advance Article
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    On-chip biosynthesis of GM1 pentasaccharide-related complex glycans

    C. S. Kim, H. R. Heo, J. H. Seo and H. J. Cha, Chem. Commun., 2019, Advance Article , DOI: 10.1039/C8CC06526H

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