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A water soluble carbazolyl-BODIPY photosensitizer with an orthogonal D–A structure for photodynamic therapy in living cells and zebrafish

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Abstract

A novel photosensitizer carbazolyl-BODIPY (Cz-BODIPY) with an orthogonal donor–acceptor structure was developed for photodynamic therapy (PDT). The photosensitizer Cz-BODIPY showed strong singlet oxygen sensitizing capability (ΦΔ = 0.68 in MeOH), excellent water solubility in dilute solution, and high photostability. The photosensitizer Cz-BODIPY exhibited negligible dark cytotoxicity and high phototoxicity (IC50 0.45 μM). Cz-BODIPY could induce cell apoptosis upon light illumination. Three cell states including living cells, apoptotic cells, and dead cells in the PDT process of Cz-BODIPY were determined via the Hoechst 33342/PI dual staining assays. The ROS (reactive oxygen species) generation in living cells during the PDT process of Cz-BODIPY was captured by the ROS detector, dihydroethidium (DHE). The photosensitizer Cz-BODIPY could be assimilated by zebrafish to generate ROS and diminish the integrity of zebrafish tissue upon light illumination. Tumor cell growth could be inhibited by Cz-BODIPY upon light illumination. The photosensitizer Cz-BODIPY displayed potential in real PDT application.

Graphical abstract: A water soluble carbazolyl-BODIPY photosensitizer with an orthogonal D–A structure for photodynamic therapy in living cells and zebrafish

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Publication details

The article was received on 23 Oct 2019, accepted on 20 Nov 2019 and first published on 22 Nov 2019


Article type: Paper
DOI: 10.1039/C9BM01709G
Biomater. Sci., 2019, Advance Article

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    A water soluble carbazolyl-BODIPY photosensitizer with an orthogonal D–A structure for photodynamic therapy in living cells and zebrafish

    C. Wang and Y. Qian, Biomater. Sci., 2019, Advance Article , DOI: 10.1039/C9BM01709G

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