Jump to main content
Jump to site search


A new lysosome-targetable fluorescent probe for detection of endogenous hydrogen polysulfides in living cells and inflamed mouse model

Author affiliations

Abstract

Hydrogen polysulfides (H2Sn, n > 1) belong to sulfane sulfur in the reactive sulfur species (RSS) family and play significant roles in maintaining biological homeostasis in organisms. The detection of H2Sn in living systems is essential, but further understanding of its “intact” function in living cells has been limited, owing to the lack of appropriate analytical tools. In this work, a new fluorescent probe PP-PS was designed for the detection of endogenous H2Sn. The probe has a large Stokes shift (178 nm), low detection limit (1 nM), and short response time (1 minute). Besides, PP-PS was successfully applied in the imaging of endogenous H2Sn in lysosomes of living cancer cells, xenograft mouse tumor tissues, and LPS-induced mouse inflammation tissues. These results revealed that the probe PP-PS could act as a new fluorescence imaging tool to study the function of intracellular hydropolysulfides.

Graphical abstract: A new lysosome-targetable fluorescent probe for detection of endogenous hydrogen polysulfides in living cells and inflamed mouse model

Back to tab navigation

Supplementary files

Publication details

The article was received on 08 Oct 2019, accepted on 20 Oct 2019 and first published on 23 Oct 2019


Article type: Paper
DOI: 10.1039/C9BM01616C
Biomater. Sci., 2020, Advance Article

  •   Request permissions

    A new lysosome-targetable fluorescent probe for detection of endogenous hydrogen polysulfides in living cells and inflamed mouse model

    L. Liang, W. Li, J. Zheng, R. Li, H. Chen and Z. Yuan, Biomater. Sci., 2020, Advance Article , DOI: 10.1039/C9BM01616C

Search articles by author

Spotlight

Advertisements