Multifunctional Theranostic Nanosystems Enabling Photothermal-Chemo Combination Therapy of Triple-Stimuli Responsive Drug Release with Magnetic Resonance Imaging
A theranostic nanosystem is emerging as a promising approach of controlled-drug delivery, diagnosis and multimodal therapeutics. Herein, a multifunctional theranostic nanoplatform is reported for photothermal-chemo combination therapy functioned with magnetic and thermal imaging. Hyaluronic acid (HA) coated Fe3O4@polydopamine nanoparticle through a redox-sensitive disulfide linker was subsequently deposited with anticancer drug doxorubicin (DOX) (termed as FPCH-DOX NPs). The nanocomposite possessed the average diameter of 120 nm, saturation magnetization of 28.5 emu g−1, DOX loading capacity of 7.13% and transverse relaxation rate of 171.76 mM−1s−1. The drug release could be triggered by pH, glutathione (GSH) and light irradiation. Prussian blue staining and confocal microscopy demonstrated the nanoplatforms could improve the biocompatibility and cellular uptake in CD44-positive HeLa cell lines rather than in CD44-negative NIH 3T3 normal cell lines. In vitro evaluations demonstrated that the combination therapy of FPCH-DOX NPs brought about as low as 16.2% cell viability, sharply lower than single chemotherapy (55.3%) or PTT (52.1%). In vivo MRI indicated the tumor accumulation of FPCH-DOX NPs by providing enhanced contrast and in vivo thermal imaging verified their localized photothermal conversion effect in tumor tissue. Importantly, FPCH-DOX NPs presented remarkable anti-tumor efficacy by photothermal-chemo combination therapy. H&E and Ki67 staining showed obvious necrosis and weak cell proliferation at the region of tumor. Thus, FPCH-DOX NPs are promising multifunctional nanoplatforms for highly effective cancer theranostics.