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Chemobrionic structures in tissue engineering: Self-assembling calcium phosphate tubes as cellular scaffolds

Abstract

A diverse range of complex patterns and mineralised hierarchical microstructures can be derived from chemobrionic systems, with formation driven by complex reaction-diffusion reactions far from thermodynamic equilibrium. In these experiments, self-assembling calcium phosphate tubes are generated using hydrogels made with 1 M calcium solutions layered with solutions of dibasic sodium phosphate over a range of concentrations between 0.2 – 1 M. Self-assembling structures prepared using 0.8 M dibasic sodium phosphate solutions were selected to assess cell-material interactions. Candidate chemobrionic scaffolds were characterised by micro- X-Ray fluorescence (µ-XRF) spectroscopy, Raman spectroscopy, powder X-ray diffraction (XRD), helium pycnometry and scanning electron microscopy (SEM). As prepared tubes were formed from non-stoichiometric hydroxyapatite (HA, Ca10-x(PO4)6-x(HPO4)x(OH)2-x (0 ⩽ x ⩽ 1)), which was confirmed as calcium deficient hydroxyapatite (CDHA, Ca9(PO4)5HPO4OH). Thermal treatment of tubes in air at 650 oC for 4 h converted the structures to beta tricalcium phosphate (β-TCP, β-Ca3(PO4)2). The potential of these scaffolds to support the attachment of bone marrow derived mesenchymal stem cells (BMSCs) was investigated for the first time, and we demonstrate cell attachment and elongation on the fabricated tubular structures.

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Publication details

The article was received on 29 Jun 2019, accepted on 27 Nov 2019 and first published on 04 Dec 2019


Article type: Paper
DOI: 10.1039/C9BM01010F
Biomater. Sci., 2019, Accepted Manuscript
  • Open access: Creative Commons BY license
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    Chemobrionic structures in tissue engineering: Self-assembling calcium phosphate tubes as cellular scaffolds

    E. A. B. Hughes, M. Chipara, T. J. Hall, R. L. Williams and L. Grover, Biomater. Sci., 2019, Accepted Manuscript , DOI: 10.1039/C9BM01010F

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