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In vivo migration of Fe3O4@polydopamine nanoparticle-labeled mesenchymal stem cells to burn injury sites and their therapeutic effects in a rat model

Abstract

Mesenchymal stem cell (MSC)-based therapy has emerged as a promising therapeutic strategy for tissue regeneration and repair. However, efficient targeted delivery to specific tissues remains an open challenge. Here, we non-invasively monitored the migration of MSC labeled with Fe3O4@polydopamine nanoparticles (Fe3O4@PDA NPs) toward laser burn injury sites in living rat and evaluated the effects of the labeled MSC at the injury site. The Fe3O4@PDA NPs could be effectively incorporated into MSC without any negative effects on stem cell properties. Furthermore, Fe3O4@PDA NPs enhanced the migration ability of the MSC by up-regulating expression level of C-X-C chemokine receptor type 4 (CXCR4). The Fe3O4@PDA NPs also increased the secretion of some cytokines and the expression of healing-related genes in comparison with unlabeled MSC. Labeled MSC was intravenously administrated into injured rat and live imaging was performed to monitor MSC migration. Fluorescent signals of labeled MSC appeared at burn injury lesions 1 day after injection and then gradually increased up to 7 days. After 7 days, the group injected with labeled MSC showed less inflammation compared with those injected with unlabeled MSC. Additionally, the labeled MSC group showed increased cytokines and reduced pro-inflammatory factors compared with the unlabeled MSC group. The Fe3O4@PDA NPs enhanced stromal cell-derived factor-1/CXCR4-mediated MSC migration in vivo. Thus, we demonstrated the safety, feasibility, and potential efficacy of using Fe3O4@PDA NPs for optimizing MSC-based therapeutic strategies for burn wound healing.

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Publication details

The article was received on 14 Feb 2019, accepted on 12 Apr 2019 and first published on 16 Apr 2019


Article type: Paper
DOI: 10.1039/C9BM00242A
Citation: Biomater. Sci., 2019, Accepted Manuscript

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    In vivo migration of Fe3O4@polydopamine nanoparticle-labeled mesenchymal stem cells to burn injury sites and their therapeutic effects in a rat model

    X. Li, Z. Wei, B. Li, J. Li, H. Lv, L. Wu, H. Zhang, B. Yang, M. Zhu and J. Jiang, Biomater. Sci., 2019, Accepted Manuscript , DOI: 10.1039/C9BM00242A

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