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Engineering a stable future for DNA-origami as a biomaterial

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Abstract

DNA as a biomaterial has evoked great interest as a potential platform for therapeutics and diagnostics and as hydrogel scaffolds due to the relative ease of programming its robust and uniform shape, site-specific functionality and controlled responsive behavior. However, for a stable self-assembled product, a relatively high cation concentration is required to prevent denaturation. Physiological and cell-culture conditions do not match these concentrations and present additional nucleases that cause a serious threat to the integrity of DNA-based materials. For the translation of this promising technology towards bioengineering challenges, stability needs to be guaranteed. Over the past years, various methods have been developed addressing the stability-related weaknesses of DNA-origami. This mini-review explains the common stability issues and compares the stabilization strategies recently developed. We present a detailed overview of each method in order to ease the selection process on which method to use for future users of DNA-origami as a biomaterial.

Graphical abstract: Engineering a stable future for DNA-origami as a biomaterial

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Publication details

The article was received on 06 Oct 2018, accepted on 28 Nov 2018 and first published on 29 Nov 2018


Article type: Minireview
DOI: 10.1039/C8BM01249K
Citation: Biomater. Sci., 2019, Advance Article
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    Engineering a stable future for DNA-origami as a biomaterial

    H. Bila, E. E. Kurisinkal and M. M. C. Bastings, Biomater. Sci., 2019, Advance Article , DOI: 10.1039/C8BM01249K

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