Issue 3, 2019

Role of ILK/p38 pathway in mediating the enhanced osteogenic differentiation of bone marrow mesenchymal stem cells on amorphous carbon coating

Abstract

Amorphous carbon (a-C) film is a promising candidate for metallic implant surface coatings to improve corrosion resistance and osteogenesis in vivo. However, the molecular mechanism of how the bioinert carbon can induce osteogenesis has not been elucidated yet. In the present study, an a-C film was deposited on a smooth pure titanium (Ti) implant surface (C-Ti) by a vacuum evaporator. The scanning electron microscopy (SEM) observation showed a homogeneous coating with dispersed granules on the C-Ti surface. Raman spectroscopy revealed the standard a-C shift bands. Rat bone marrow mesenchymal stem cells (MSCs) were cultured on different implant surfaces. In agreement with other publications, the C-Ti surface exhibited enhanced cell adhesion, proliferation and osteogenic differentiation. In addition, a western blot analysis of the mitogen-activated protein kinase (MAPK) signaling pathways revealed that only the p38 phosphorylation level was increased significantly on the C-Ti surface. Inhibition of p38 by SB203580 obliterated the enhanced osteogenic differentiation ability of the C-Ti surface. Furthermore, integrin-linked kinase (ILK) was significantly upregulated on the C-Ti surface during the initial three days. Specific knockdown of ILK by siRNA sharply decreased p38 phosphorylation and also resulted in reduced osteogenic differentiation on the C-Ti surface. Above all, our study indicates that the a-C coating is able to promote osteogenic differentiation of MSCs through the ILK/p38 signaling pathway.

Graphical abstract: Role of ILK/p38 pathway in mediating the enhanced osteogenic differentiation of bone marrow mesenchymal stem cells on amorphous carbon coating

Article information

Article type
Paper
Submitted
18 Sep 2018
Accepted
16 Nov 2018
First published
12 Dec 2018

Biomater. Sci., 2019,7, 975-984

Role of ILK/p38 pathway in mediating the enhanced osteogenic differentiation of bone marrow mesenchymal stem cells on amorphous carbon coating

G. Yue, W. Song, S. Xu, Y. Sun and Z. Wang, Biomater. Sci., 2019, 7, 975 DOI: 10.1039/C8BM01151F

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements