Jump to main content
Jump to site search


A quadruple-label time-resolved fluorescent immunochromatographic assay for simultaneous quantitative determination of three mycotoxins in grains

Abstract

A multiple quadruple-label time-resolved fluorescent immunochromatographic assay (QL-TRFICA) for simultaneous quantitative detecting aflatoxin B1 (AFB1), zearalenone (ZEN) and ochratoxin A (OTA) in grains was established in this paper. This assay was developed based on quadruple-label immunoprobes, one of which was a chicken IgY-specific antibody coupled with time-resolved fluorescent nanobeads (TRFN) for the test C line signal while the other three were specific to AFB1, ZEN and OTA monoclonal antibodies coupled with TRFN for the test T line signal. Quantitative relationships between three mycotoxins concentrations and T/C ratios were established to determine the analyte concentration through a portable multi-channel fluorescence reader. The limits of detection (LODs) for AFB1, ZEN and OTA were 0.04 μg kg-1, 0.20 μg kg-1 and 0.10 μg kg-1 in 6 grains (corn, soybean, sorghum, wheat, rice and oats), respectively. Recoveries ranged from 71.60% to 119.98% at fortified concentration of LOD, 2LOD and 4LOD, with coefficient of variation <15%. Analysis of field grain samples by QL-TRFICA was in accordance with that of LC-MS/MS. Therefore, this proposed multiplex QL-TRFICA was a promising method for on-site screening of multiple mycotoxins in grains.

Back to tab navigation

Supplementary files

Publication details

The article was received on 26 Oct 2019, accepted on 27 Nov 2019 and first published on 27 Nov 2019


Article type: Paper
DOI: 10.1039/C9AY02309G
Anal. Methods, 2019, Accepted Manuscript

  •   Request permissions

    A quadruple-label time-resolved fluorescent immunochromatographic assay for simultaneous quantitative determination of three mycotoxins in grains

    X. Chang, Y. Zhang, H. Liu and X. Tao, Anal. Methods, 2019, Accepted Manuscript , DOI: 10.1039/C9AY02309G

Search articles by author

Spotlight

Advertisements