Jump to main content
Jump to site search


Development of disk-shaped monolithic microplates for detecting multiple mycotoxins

Abstract

Organic polymer monoliths have been used extensively for separation and purification of molecules because of their excellent mass transfer properties and versatile surface modification. However, the organic polymer monolithic materials have the significant UV adsorption, which blocked UV-visible absorbance detection. Thus, we developed a novel disk-shaped mycotoxin immune-affinity monolithic microplate for quantification detection of mycotoxins. The concentric disk-shaped format allow the obscure organic monoliths capture mycotoxins and unobstructed UV-visible absorbance measurement performed in the same microplates. The limits of detection of 96 disk-shaped mycotoxin immune-affinity monolithic microplates were 161.73, 22.52, 0.14, and 0.21 μg·Kg-1 for Deoxynivalenol (DON), Zearalenone (ZEN), Aflatoxin B1 (AFB1), and Ochratoxin A (OTA), respectively, which meet well the requirements for detection of foodstuffs. The recovery rate ranges of the four mycotoxins were 87%-92%, 82%-89%, 78%-116%, and 83%-87% for DON, ZEN, AFB1, and OTA disk-shaped microplate, respectively, which proved that the disk-shaped monolithic microplates have good reproducibility for mycotoxin detection. In summary, the 96 disk-shaped mycotoxin immune-affinity monolithic microplate is a sensitive, accurate, stable, and convenient platform for detection of mycotoxin in agro-food.

Back to tab navigation

Supplementary files

Publication details

The article was received on 11 Oct 2019, accepted on 01 Nov 2019 and first published on 04 Nov 2019


Article type: Paper
DOI: 10.1039/C9AY02194A
Anal. Methods, 2019, Accepted Manuscript

  •   Request permissions

    Development of disk-shaped monolithic microplates for detecting multiple mycotoxins

    H. Wang, Y. Yang, Y. Wang, S. Zhang and L. Li, Anal. Methods, 2019, Accepted Manuscript , DOI: 10.1039/C9AY02194A

Search articles by author

Spotlight

Advertisements