Development of disk-shaped monolithic microplates for detecting multiple mycotoxins
Organic polymer monoliths have been used extensively for separation and purification of molecules because of their excellent mass transfer properties and versatile surface modification. However, the organic polymer monolithic materials have the significant UV adsorption, which blocked UV-visible absorbance detection. Thus, we developed a novel disk-shaped mycotoxin immune-affinity monolithic microplate for quantification detection of mycotoxins. The concentric disk-shaped format allow the obscure organic monoliths capture mycotoxins and unobstructed UV-visible absorbance measurement performed in the same microplates. The limits of detection of 96 disk-shaped mycotoxin immune-affinity monolithic microplates were 161.73, 22.52, 0.14, and 0.21 μg·Kg-1 for Deoxynivalenol (DON), Zearalenone (ZEN), Aflatoxin B1 (AFB1), and Ochratoxin A (OTA), respectively, which meet well the requirements for detection of foodstuffs. The recovery rate ranges of the four mycotoxins were 87%-92%, 82%-89%, 78%-116%, and 83%-87% for DON, ZEN, AFB1, and OTA disk-shaped microplate, respectively, which proved that the disk-shaped monolithic microplates have good reproducibility for mycotoxin detection. In summary, the 96 disk-shaped mycotoxin immune-affinity monolithic microplate is a sensitive, accurate, stable, and convenient platform for detection of mycotoxin in agro-food.