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Issue 44, 2019
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A folic acid modified polystyrene nanosphere surface for circulating tumor cell capture

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Abstract

Circulating tumor cells (CTCs) have been considered as a significant biomarker for cancer metastasis and relapse. Effective detection of CTCs will play an important role in early cancer diagnosis, prognosis, progress monitoring, and personalized therapy. In this work, we developed a polystyrene (PS) nanosphere substrate to capture CTCs from whole blood samples. Bovine serum albumin (BSA), as an antifouling molecule, and folic acid, as a recognizing agent, were modified onto the surface of PS nanospheres via self-polymerization of dopamine. 8 HeLa cells could be captured when 10 cells were spiked into 1 mL of the whole blood sample. Then, the captured target cells were further proliferated through an in situ culture strategy on the polystyrene (PS) nanosphere substrate, and nearly 80 HeLa cells were obtained from 1 captured cell after 7 days of in situ culture, revealing the potential application for CTC isolation and proliferation.

Graphical abstract: A folic acid modified polystyrene nanosphere surface for circulating tumor cell capture

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Publication details

The article was received on 25 Sep 2019, accepted on 10 Oct 2019 and first published on 14 Oct 2019


Article type: Paper
DOI: 10.1039/C9AY02090J
Anal. Methods, 2019,11, 5718-5723

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    A folic acid modified polystyrene nanosphere surface for circulating tumor cell capture

    C. Chen, Z. Wang, Y. Zhao, Y. Cao, P. Ding, H. Liu, N. Su and R. Pei, Anal. Methods, 2019, 11, 5718
    DOI: 10.1039/C9AY02090J

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