Jump to main content
Jump to site search


A novel ratiometric fluorescent probe for highly sensitive and selective detection of peroxynitrite and its application for tracing endogenous peroxynitrite in live cells

Author affiliations

Abstract

Peroxynitrite (ONOO) is a reactive and short-lived biological oxidant endogenously generated in biosystems, and its excessive production is related to various diseases. However, the functional mechanisms of ONOO are not fully understood. Here, we present a novel ratiometric fluorescent probe CPD-ratio for the accurate detection of ONOO under single wavelength excitation. This study showed that the probe CPD-ratio can achieve the ratiometric detection of ONOO with rapid response, and it possessed excellent selectivity and sensitivity (detection limit was calculated to be 47 nM). These properties and its non-toxicity demonstrated that probe CPD-ratio could be potentially employed to accurately monitor ONOO levels in biological systems. We have thoroughly demonstrated the utility of the probe CPD-ratio for ratiometric bioimaging, because it has been effectively used to trace the exogenous and endogenous ONOO in live cells.

Graphical abstract: A novel ratiometric fluorescent probe for highly sensitive and selective detection of peroxynitrite and its application for tracing endogenous peroxynitrite in live cells

Back to tab navigation

Supplementary files

Publication details

The article was received on 23 Sep 2019, accepted on 14 Oct 2019 and first published on 14 Oct 2019


Article type: Paper
DOI: 10.1039/C9AY02069A
Anal. Methods, 2019, Advance Article

  •   Request permissions

    A novel ratiometric fluorescent probe for highly sensitive and selective detection of peroxynitrite and its application for tracing endogenous peroxynitrite in live cells

    Z. Li, C. Yu, Y. Chen, C. Liu, P. Jia, H. Zhu, X. Zhang, W. Sheng and B. Zhu, Anal. Methods, 2019, Advance Article , DOI: 10.1039/C9AY02069A

Search articles by author

Spotlight

Advertisements