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Fe3O4/PEDOT:PSS nanocomposite as an advanced electroconductive material for biosensing of prostate specific antigen in unprocessed human plasma samples

Abstract

A novel sandwich-type biosensor based on signal amplification strategy of various nanocomposites was developed to monitoring of prostate specific antigen (PSA) in real samples. The engineered immunosensor fabricated by iron oxide magnetic nanoparticles modified polymer poly (3,4-ethylenedioxythiophene): poly (styrene sulfonate) [Fe3O4/PEDOT.PSS] conductive nanocomposite that utilized to immobilization of primary antibodies (biotinylated antibody (Ab1)). In order to improve electron transfer rate, cysteamine caped gold nanoparticle (AuNPs-Cys A) was applied to conjugation with secondary antibody (HRP-Ab2). All of the fabrication procedure was investigated by square wave voltammetry technique. Under optimized experimental condition, the calibration curve showed wide linear range (0.001-0.5 µg/L) for PSA biomarker with low limit of quantification 0.001 µg/L. Finally, satisfactory results were obtained for the determination of PSA in untreated human plasma samples and indicating the potential of the immunoassay to be applied in clinical bioanalysis.

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Publication details

The article was received on 13 Sep 2019, accepted on 05 Oct 2019 and first published on 07 Oct 2019


Article type: Paper
DOI: 10.1039/C9AY01988J
Anal. Methods, 2019, Accepted Manuscript

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    Fe3O4/PEDOT:PSS nanocomposite as an advanced electroconductive material for biosensing of prostate specific antigen in unprocessed human plasma samples

    M. Shaban, M. Hasanzadeh and E. Solhi, Anal. Methods, 2019, Accepted Manuscript , DOI: 10.1039/C9AY01988J

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