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A one-step synthesis of novel high pH-sensitive nitrogen-doped yellow fluorescent carbon dots and their detection application in living cells

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Abstract

Monitoring the pH in living cells is of great significance for a deeper understanding of cellular functions for effective disease diagnosis. Herein, a rare example of N-doped carbon dots (N-CDs) that emit bright-yellow fluorescence prepared using o-phenylenediamine as a carbon precursor via a simple one-pot hydrothermal method is reported. The as-prepared N-CDs exhibited not only highly optical stability and good biocompatibility but also pH sensitivity. A good linear relationship between the fluorescence intensity and the pH value could be obtained in the range of 4.2 to 7.0 (R2 = 0.9983). In addition, N-CDs also possess superior selectivity toward hydrogen ions over common metal ions and some bioactive molecules. On this basis, N-CDs are successfully applied to cellular imaging and to monitor pH fluctuations in living cells.

Graphical abstract: A one-step synthesis of novel high pH-sensitive nitrogen-doped yellow fluorescent carbon dots and their detection application in living cells

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Publication details

The article was received on 20 Aug 2019, accepted on 16 Sep 2019 and first published on 10 Oct 2019


Article type: Paper
DOI: 10.1039/C9AY01794A
Anal. Methods, 2019, Advance Article

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    A one-step synthesis of novel high pH-sensitive nitrogen-doped yellow fluorescent carbon dots and their detection application in living cells

    L. Shen, C. Hou, J. Li, X. Wang, Y. Zhao, H. Yang, S. Yang, M. Yang, X. Luo and D. Huo, Anal. Methods, 2019, Advance Article , DOI: 10.1039/C9AY01794A

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