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Chemical derivatization coupled with matrix assisted laser desorption ionization time-of-flight mass spectrometry for convenient and efficient N-glycan detection in human plasma

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Abstract

As one of the most common post-translational modifications, protein N-glycosylation is involved in most important biological processes. Structural changes of the protein linked N-glycans have close correlations with the occurrence and progression of various diseases. Therefore, efficient detection of these disease-associated aberrations is of great significance for the development of new diagnostic biomarkers and therapeutic drug targets. In this work, we developed a method for convenient and effective N-glycan detection by coupling condition controlled chemical derivatization with MALDI-TOF MS. Due to the high reaction efficiency and enhanced hydrophobicity, improved identification scales were obtained for N-glycans from both asialofetuin and human plasma compared with the direct analysis.

Graphical abstract: Chemical derivatization coupled with matrix assisted laser desorption ionization time-of-flight mass spectrometry for convenient and efficient N-glycan detection in human plasma

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Publication details

The article was received on 13 Aug 2019, accepted on 20 Oct 2019 and first published on 21 Oct 2019


Article type: Paper
DOI: 10.1039/C9AY01730E
Anal. Methods, 2019, Advance Article

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    Chemical derivatization coupled with matrix assisted laser desorption ionization time-of-flight mass spectrometry for convenient and efficient N-glycan detection in human plasma

    H. H. Bai, J. Liu, X. Q. Cheng and X. H. Wang, Anal. Methods, 2019, Advance Article , DOI: 10.1039/C9AY01730E

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