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Nitrogen and phosphorus co-doped carbon dots as a “turn-off-on” fluorescence probe for the detection of Hg2+ and GSH and cell imaging

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Abstract

Nitrogen and phosphorus co-doped carbon dots (NPCDs) were synthesized by a microwave method, using citric acid monohydrate and diammonium hydrogen phosphate as raw materials. The morphology, structures and elemental compositions of NPCDs were characterized by transmission electron microscopy, X-ray diffractometry, and X-ray photoelectron spectroscopy. It was found that NPCDs show good selectivity to Hg2+ over other metal ions. And the fluorescence of the NPCDs-Hg2+ system can be recovered in the presence of glutathione (GSH). As such, a “turn-off-on” fluorescence probe can be constructed for the detection of Hg2+ and GSH. Under the optimum working conditions, the linear response of NPCDs to Hg2+ was in the range 0.10–30 μM with a detection limit of 9.9 nM. The linear range of GSH was 0.50–32 μM with a limit of detection of 11.2 nM. The proposed method has been successfully applied to the detection of Hg2+ and GSH in various biological samples. What is more, NPCDs have low cytotoxicity verified by the MTT assay. Using NPCDs as a probe, fluorescence images were obtained for HepG2 cells, which indicated that it could monitor the levels of Hg2+ and GSH in biological systems.

Graphical abstract: Nitrogen and phosphorus co-doped carbon dots as a “turn-off-on” fluorescence probe for the detection of Hg2+ and GSH and cell imaging

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Publication details

The article was received on 10 Aug 2019, accepted on 07 Oct 2019 and first published on 18 Oct 2019


Article type: Paper
DOI: 10.1039/C9AY01723B
Anal. Methods, 2019, Advance Article

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    Nitrogen and phosphorus co-doped carbon dots as a “turn-off-on” fluorescence probe for the detection of Hg2+ and GSH and cell imaging

    X. Yang, M. Zhang, Y. Zhang, N. Wang, W. Bian and M. M. F. Choi, Anal. Methods, 2019, Advance Article , DOI: 10.1039/C9AY01723B

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