Jump to main content
Jump to site search


Radial multi-stationary phase thin-layer chromatography for the field-ready fingerprinting of herbal materials

Author affiliations

Abstract

The growing international demand for herbal-based products has prompted the need for more stringent quality control methods to detect substandard and adulterated herbal plant materials. However, most prescreening methods, such as thin-layer chromatography (TLC), remain inaccessible to herbal producers in resource-limited settings. Here, we report a proof-of-concept demonstration of a multi-stationary phase thin-layer chromatography (MSP-TLC) method for the preliminary on-site identification and evaluation of herbal materials. Our method is based on a unique TLC plate design that features multiple phenyl- and octyl-modified silica gel stationary phases configured as radial sectors. The modified stationary phase patterns were fabricated by screen-printing organosilane solutions onto commercial silica gel TLC plates. Radial elution with an ethanol–water mobile phase from the center of the MSP-TLC plate generates multiple chromatographic profiles simultaneously for a single sample extract. To facilitate the interpretation of the multiple TLC profiles, the MSP-TLC system was coupled with image analysis and chemometric pattern recognition to classify a sample as “within-specifications” or “off-specifications” for a given herbal plant species. Application of the system to Blumea balsamifera and Vitex negundo demonstrated sensitivity and specificity rates that range from 73.1 to 95.1% compared to the respective standard Pharmacopeia TLC methods. The presented method holds considerable promise as a cost-effective, user-friendly on-site prescreening tool for herbal materials in resource-limited settings.

Graphical abstract: Radial multi-stationary phase thin-layer chromatography for the field-ready fingerprinting of herbal materials

Back to tab navigation

Supplementary files

Publication details

The article was received on 09 Aug 2019, accepted on 28 Sep 2019 and first published on 07 Oct 2019


Article type: Paper
DOI: 10.1039/C9AY01714C
Anal. Methods, 2019, Advance Article

  •   Request permissions

    Radial multi-stationary phase thin-layer chromatography for the field-ready fingerprinting of herbal materials

    S. M. Sibug-Torres, I. D. Padolina and E. P. Enriquez, Anal. Methods, 2019, Advance Article , DOI: 10.1039/C9AY01714C

Search articles by author

Spotlight

Advertisements