Jump to main content
Jump to site search


A rapid tag-free identification of Escherichia coli antibiotic-resistant isolates using Raman scattering

Author affiliations

Abstract

Rapid and accurate detection of pathogenic bacteria is a critical issue for public health and food safety. We introduce a rapid tag-free identification method for subtyping clinically relevant human blood-cultured Escherichia coli (E. coli) colonies. Gold-deposited surface-enhanced Raman scattering (SERS)-functionalized substrates were used to identify seven E. coli isolates. The method achieved a rapid analysis time of 30 min, an enhancement factor of 1.6 × 104 to Raman intensities, and high reproducibility with approximately 5% relative standard deviation. The proposed SERS procedure coupled with a multivariate statistics-supported classification method showed good performance compared to previous procedures. Therefore, the SERS-based tag-free identification method has high potential for rapidly subtyping clinically relevant quinolone-resistant E. coli antibiotic-resistant isolates. In future, the SERS-based biosensing technique may be applied in the clinical laboratory using a portable Raman machine and optic probe Raman system.

Graphical abstract: A rapid tag-free identification of Escherichia coli antibiotic-resistant isolates using Raman scattering

Back to tab navigation

Supplementary files

Publication details

The article was received on 09 Aug 2019, accepted on 01 Oct 2019 and first published on 01 Oct 2019


Article type: Paper
DOI: 10.1039/C9AY01713E
Anal. Methods, 2019, Advance Article

  •   Request permissions

    A rapid tag-free identification of Escherichia coli antibiotic-resistant isolates using Raman scattering

    S. Kim, S. H. Lee, Y. J. Kim, H. J. Lee and S. Choi, Anal. Methods, 2019, Advance Article , DOI: 10.1039/C9AY01713E

Search articles by author

Spotlight

Advertisements