Jump to main content
Jump to site search


Electrochemical detection of aflatoxin B1: an aptasensor prepared using graphene oxide and gold nanowires

Author affiliations

Abstract

An electrochemical aptasensor was developed for the detection of aflatoxin B1 using a nanocomposite of graphene oxide and gold nanowires. The aptasensor mechanism was based on the difference in the differential pulse voltammetry peak current upon detachment of the aptamer from the electrode surface when the toxin is present. The working electrode modifications were confirmed using field emission scanning electron microscopy, energy-dispersive X-ray spectroscopy, and electrochemical methods cyclic voltammetry and electrochemical impedance spectroscopy. The limit of detection was 1.4 pM and the linear range was 5.0–750.0 pM with good reproducibility of the results. The specificity test of the aptasensor successfully confirmed that the aptasensor is specific towards aflatoxin B1 compared to a blank solution and non-specific molecules. A simulated real sample environment tested in pistachio samples alongside experiments and comparison with the HPLC method confirmed the aptasensor's capability to work in future real food assays.

Graphical abstract: Electrochemical detection of aflatoxin B1: an aptasensor prepared using graphene oxide and gold nanowires

Back to tab navigation

Publication details

The article was received on 05 Aug 2019, accepted on 28 Oct 2019 and first published on 30 Oct 2019


Article type: Paper
DOI: 10.1039/C9AY01673B
Anal. Methods, 2019, Advance Article

  •   Request permissions

    Electrochemical detection of aflatoxin B1: an aptasensor prepared using graphene oxide and gold nanowires

    S. Mousavi Nodoushan, N. Nasirizadeh, R. Kachuei and A. A. Imani Fooladi, Anal. Methods, 2019, Advance Article , DOI: 10.1039/C9AY01673B

Search articles by author

Spotlight

Advertisements