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A perylenediimide based ‘on-off’ chemosensor for the detection of nucleoside triphosphates: an efficient ensemble for monitoring alkaline phosphatase activity

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Abstract

The evaluation of the optical properties of BAB-PDI towards nucleoside polyphosphates (NPPs) in 90% aqueous media (pH 7.2) revealed that upon addition of guanosine triphosphate (GTP), the fluorescence intensity of BAB-PDI was >85% quenched. In the case of adenosine triphosphate (ATP), uridine triphosphate (UTP) and cytidine triphosphate (CTP), only 30–65% quenching of fluorescence intensity was observed. Other NPPs such as nucleoside diphosphates and monophosphates, respectively, caused 20–30% and <5% quenching of fluorescence intensity of BAB-PDI due to fewer negative charges on di- and mono-phosphates. The minimum limit of detection of GTP reached 19.3 nM. The application of BAB-PDI in monitoring enzyme (alkaline phosphatase) activity in aqueous solution using a BAB-PDI + GTP ensemble has also been demonstrated.

Graphical abstract: A perylenediimide based ‘on-off’ chemosensor for the detection of nucleoside triphosphates: an efficient ensemble for monitoring alkaline phosphatase activity

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Publication details

The article was received on 28 Jul 2019, accepted on 30 Sep 2019 and first published on 01 Oct 2019


Article type: Paper
DOI: 10.1039/C9AY01608B
Anal. Methods, 2019, Advance Article

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    A perylenediimide based ‘on-off’ chemosensor for the detection of nucleoside triphosphates: an efficient ensemble for monitoring alkaline phosphatase activity

    L. S. Mittal, P. Sharma, N. Kaur and P. Singh, Anal. Methods, 2019, Advance Article , DOI: 10.1039/C9AY01608B

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