Development of an immunochromatographic strip for the detection of rosiglitazone in functional foods based on monoclonal antibodies

Abstract

An anti-rosiglitazone (RSG) monoclonal antibody (4G6) was prepared to develop an immunochromatographic strip for the detection of illicitly added RSG in hypoglycemic functional foods. The monoclonal antibody was highly specific for RSG, with a half-life inhibitory concentration (IC₅₀) of 1.0889 ng mL⁻¹. Its limit of detection was 0.2178 ng mL⁻¹ with a linear detection range of 0.2178–1.742 ng mL⁻¹. The cut-off values of colloidal gold-based immunochromatographic test strips in phosphate buffered saline and functional food samples were both 10 ng mL⁻¹. In the spiked samples and recovery tests, the recovery ranged from 96.58% to 101.68%. The results obtained by the immunochromatographic assay were consistent with those obtained by indirect competitive-ELISA. Therefore, our developed immunochromatographic strip system is suitable for the on-site detection and rapid screening of RSG in food samples.