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Systematic investigation of a quantitative paper-based DNA reader (qPDR) for distance-based quantification of nucleic acids and mercury ions

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Abstract

Nucleic acid testing using microfluidic paper-based analytical devices (μPAD) offers exciting opportunities for inexpensive, portable diagnostics in resource-limited settings. We have previously developed a portable, affordable, and easy-to-fabricate quantitative paper-based DNA reader (qPDR) capable of quantifying nucleic acids by measuring distance as a digital readout. Here, to decipher specific molecular interactions and identify optimal experimental conditions for using qPDR, we systematically investigated the chromatographic behaviours of SYBR Green I (SG), a key assay component, in the presence of DNA, organic solvents, saline, urea, and surfactants. We also demonstrated the possibility to expand qPDR for the detection of non-nucleic-acid targets. Specifically, we have successfully enabled distance-based quantification of mercury ions with nanomolar detection limit using thymine-rich DNA and qPDR.

Graphical abstract: Systematic investigation of a quantitative paper-based DNA reader (qPDR) for distance-based quantification of nucleic acids and mercury ions

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Publication details

The article was received on 01 Jul 2019, accepted on 06 Oct 2019 and first published on 07 Oct 2019


Article type: Paper
DOI: 10.1039/C9AY01383K
Anal. Methods, 2019, Advance Article

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    Systematic investigation of a quantitative paper-based DNA reader (qPDR) for distance-based quantification of nucleic acids and mercury ions

    T. Dong, G. A. Wang, M. W. Li and F. Li, Anal. Methods, 2019, Advance Article , DOI: 10.1039/C9AY01383K

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