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Issue 31, 2019
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An automated high-throughput sample preparation method using double-filtration for serum metabolite LC-MS analysis

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Abstract

The rapid and reproducible preparation of biological samples is an essential requirement for LC-MS-based clinical metabolomic profiling. Herein, we proposed a novel, automated sample preparation method for serum metabolite LC-MS analysis. This method included a one-step double-filtration process with two filters (30k and 10k MWCO). High sample throughput (up to 96 samples) was achieved in an automated manner by incorporating the process in a liquid handling robotic system. The effectiveness of the developed method was evaluated through comparison with a conventional manual centrifugation-based method using seven human serum samples. A higher metabolite recovery and better reproducibility were achieved in quantitative analysis using the automated double-filtration method. The results suggested that the multiplexed serum sample preparation system can be applied to large-scale untargeted metabolomics studies.

Graphical abstract: An automated high-throughput sample preparation method using double-filtration for serum metabolite LC-MS analysis

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Supplementary files

Article information


Submitted
29 May 2019
Accepted
03 Jul 2019
First published
04 Jul 2019

Anal. Methods, 2019,11, 4060-4065
Article type
Technical Note

An automated high-throughput sample preparation method using double-filtration for serum metabolite LC-MS analysis

M. Joo, J. Park, V. Duong, D. Kwon, J. Jeon, M. Han, B. Cho, H. Choi, C. Lee, H. Kang and H. Lee, Anal. Methods, 2019, 11, 4060
DOI: 10.1039/C9AY01137D

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