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Graphene oxide-circular aptamer based colorimetric protein detection on bioactive paper

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Abstract

Paper-based sensor technology represents a new class of point-of-care (POC) diagnostic devices that is affordable, portable, rapid and scalable for manufacturing. One of the goals of developing new paper-based POC diagnostics is the need to recognize targets with high specificity and sensitivity. Herein, a graphene oxide-circular aptamer based assay is described for highly specific and sensitive detection of a target protein on bioactive paper. The circular aptamer was self-assembled onto graphene oxide to generate the biorecognition moieties, followed by the desorption reaction induced by the specific binding of the target. The released circular aptamer then hybridizes to paper-bound DNA primers, thus initiating the rolling circle amplification reaction to produce a long DNA molecule containing multiple horseradish peroxidase-mimicking DNAzyme units, which further catalyze the oxidation of substrates by H2O2 in the presence of hemin to yield a distinct colorimetric signal on paper. Under the optimal conditions, this dual amplification method can achieve detection of platelet-derived growth factor (PDGF) at a concentration as low as 100 pM. Overall, this strategy of paper-based nanobiosensors may hold great potential for rapid, accurate and inexpensive biomarker detection in POC applications.

Graphical abstract: Graphene oxide-circular aptamer based colorimetric protein detection on bioactive paper

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Publication details

The article was received on 21 May 2019, accepted on 12 Jul 2019 and first published on 12 Jul 2019


Article type: Paper
DOI: 10.1039/C9AY01060B
Anal. Methods, 2019, Advance Article

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    Graphene oxide-circular aptamer based colorimetric protein detection on bioactive paper

    X. Li, X. He, Q. Zhang, Y. Chang and M. Liu, Anal. Methods, 2019, Advance Article , DOI: 10.1039/C9AY01060B

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