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Application of quantitative spectral deconvolution of 1H NMR (qsd-NMR) in the simultaneous quantitative determination of creatinine and metformin in the human urine

Abstract

Determination of residual creatinine and metformin in the urine is essential in estimating the creatinine clearance and monitoring the therapeutic effect of metformin, respectively, in type 2 diabetes mellitus (T2DM) patients. This report describes the validation of the quantitative spectral deconvolution of 1H NMR method (qsd-NMR) and its application in simultaneous determination of the creatinine and metformin concentrations in the human urine. Two different approaches, namely, the single-individual and the mixture of standards, were successfully developed and validated against the referenced concentrations. The method was subsequently tested for the determination of the two analytes in the healthy human urine spiked with metformin. The results showed that the method was selective with high linearity, accuracy, and precision. The method based on the single-individual standard approach was more suitable for creatinine, while that based on the mixture of standards was more suitable for metformin quantitative determinations. The results suggested that the method is potentially useful in the determination of creatinine, and metformin concentration monitoring of the urine in T2DM patient undergoing metformin treatment.

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Publication details

The article was received on 22 Mar 2019, accepted on 23 Sep 2019 and first published on 25 Sep 2019


Article type: Technical Note
DOI: 10.1039/C9AY00594C
Anal. Methods, 2019, Accepted Manuscript

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    Application of quantitative spectral deconvolution of 1H NMR (qsd-NMR) in the simultaneous quantitative determination of creatinine and metformin in the human urine

    M. Maulidiani, F. Abas, R. Rudiyanto, N. M. H. Abdullah, A. Azlan and N. H. Lajis, Anal. Methods, 2019, Accepted Manuscript , DOI: 10.1039/C9AY00594C

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