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Issue 23, 2019
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A two-photon fluorescent probe for imaging of endogenous formaldehyde in HeLa cells and quantitative detection of basal formaldehyde in milk samples

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Abstract

Herein, a two-photon fluorescent probe NaP for formaldehyde (FA) was rationally constructed and reported. The probe presented favorable properties responding to FA, such as a remarkable ∼29-fold fluorescence signal enhancement at 550 nm, good selectivity, high sensitivity and a low detection limit which was calculated to be 1.62 × 10−6 M. Furthermore, the probe NaP was membrane-permeable and successfully employed for visualization of endogenous FA in living cells. Meanwhile, the FA content in six milk samples was quantitatively detected and the accuracy was assessed by the 3-methyl-2-benzothiazolinone hydrazone (MBTH) method, which is a standard UV spectrophotometric method. As expected, the proposed probe NaP may serve as a promising tool for imaging of endogenous FA in living systems and quantitative detection of FA in the food industry.

Graphical abstract: A two-photon fluorescent probe for imaging of endogenous formaldehyde in HeLa cells and quantitative detection of basal formaldehyde in milk samples

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Supplementary files

Article information


Submitted
18 Mar 2019
Accepted
20 May 2019
First published
20 May 2019

Anal. Methods, 2019,11, 2969-2975
Article type
Paper

A two-photon fluorescent probe for imaging of endogenous formaldehyde in HeLa cells and quantitative detection of basal formaldehyde in milk samples

F. Xin, Y. Tian, J. Jing and X. Zhang, Anal. Methods, 2019, 11, 2969
DOI: 10.1039/C9AY00553F

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