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Issue 18, 2019
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A fluorescent signal-on sensor based on photoinduced electron transfer between copper nanoparticles and G-quadruplex/hemin

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Abstract

A novel fluorescence signal-on biosensing platform was developed by using photoinduced electron transfer (PET) between copper nanoclusters (CuNCs) and G-quadruplex/hemin. Hairpin DNA was chosen as an effective template to synthesize CuNCs. A split G-quadruplex sequence is linked to the end of the hairpin DNA template. In the presence of K+ and hemin, the split G-quadruplex sequence can form a G-quadruplex/hemin complex and quench the fluorescence of CuNCs via PET. Target recognition causes split G-quadruplexes to move away from each other, and prevents the formation of a G-quadruplex/hemin complex. Thus, a significant fluorescence signal increase is observed, because of the lower PET efficiency. This strategy is successfully applied for the detection of DNA. Otherwise, it can be used for developing a platform for detection of small molecules and proteins.

Graphical abstract: A fluorescent signal-on sensor based on photoinduced electron transfer between copper nanoparticles and G-quadruplex/hemin

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Supplementary files

Article information


Submitted
05 Mar 2019
Accepted
10 Apr 2019
First published
16 Apr 2019

Anal. Methods, 2019,11, 2410-2414
Article type
Paper

A fluorescent signal-on sensor based on photoinduced electron transfer between copper nanoparticles and G-quadruplex/hemin

L. J. Ou, S. W. Qin, H. Bo, J. X. Luo, N. C. Yuan, A. M. Sun, L. Y. Wang and W. H. Liu, Anal. Methods, 2019, 11, 2410
DOI: 10.1039/C9AY00461K

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