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Fluorescent signal-on sensor based on photoinduced electron transfer between copper nanoparticles and G-quadruplex/hemin

Abstract

A novel fluorescence signal-on biosensing platform was developed by using photoinduced electron transfer (PET) between copper nanoclusters (CuNCs) and G-quadruplex/hemin. Haipin DNA was choosed as the effective template to synthesize CuNCs. Split G-quadruplex sequence is linked to the end of haipin DNA template. In the presence of K+ and hemin, split G-quadruplex sequence can form the G-quadruplex/hemin complex and quenched the fluorescence of CuNCs via PET. Target recognition induced the split G-quadruplex being away from each other, that prevents the formation of G-quadruplex/hemin complex. Thus, a significant fluorescence signal increasing is observed, because of the lower PET efficiency. This strategy is successfully applied for the detection of DNA. Otherwise, it can be used for developing platform for small moluculer and protein.

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Publication details

The article was received on 05 Mar 2019, accepted on 10 Apr 2019 and first published on 16 Apr 2019


Article type: Paper
DOI: 10.1039/C9AY00461K
Citation: Anal. Methods, 2019, Accepted Manuscript

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    Fluorescent signal-on sensor based on photoinduced electron transfer between copper nanoparticles and G-quadruplex/hemin

    L. Ou, S. Qin, H. Bo, J. Luo, N. Yuan, M. A. Sun, L. Wang and H. Liu, Anal. Methods, 2019, Accepted Manuscript , DOI: 10.1039/C9AY00461K

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