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Analysis of neurochemicals by capillary electrophoresis in athlete’s urine and a pilot study of their changes responding to sport fatigue

Abstract

Neurochemicals may play an important role in metabolism and sport fatigue. In order to investigate the potential links, a simple, rapid, accurate analytical method, capillary zone electrophoresis (CZE) mode capable of carrying out a simultaneous separation of 11 neurochemicals has been developed and validated. The 11 analytes included neurotransmitter precursors : arginine(Arg), tyrosine, neurotransmitters: serotonin(5-HT), dopamine (DA), epinephrine (EP), neurotransmitter metabolites : 5-hydroxy-3-indoleacetic acid (5-HIAA), homovanillic acid (HVA), vanilla mandelic acid (VMA), and matrix composition of urine: creatinine (Cre), uric acid, acetoacetic acid. These could be well separated within 7 min at a separation voltage of +25 kV and select 12.5 mmol/L Na2B4O7 (pH=9.9) as running buffer. The proposed method allowed the limits of detections of urinary metabolites to be in the range of 0.04~1.50 μmol/L (S/N=3) and the RSDs of migration time were 0.09~0.91%. For three models of physiological status: normal, fatigue, relaxed; to establish an athlete model of fatigue, the athletes continued to ride a bike for 45 minutes, we selected the RPE1 (Ratings of perceived exertion ) scale for evaluation of the extent of fatigue, the RPE values are more than 18 in model of fatigue of 5 athletes. In this study, we found Arg was lower 10.6~62.2 % in urine of sport fatigue compared with normal and relaxed states and the excretion of HVA increased 2.75~36.42 % after sport fatigue. This method can be used for potential applications involving the determination of two biomarker compounds for the rapid diagnosis of fatigue models.

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Publication details

The article was received on 05 Mar 2019, accepted on 10 Apr 2019 and first published on 12 Apr 2019


Article type: Paper
DOI: 10.1039/C9AY00457B
Citation: Anal. Methods, 2019, Accepted Manuscript

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    Analysis of neurochemicals by capillary electrophoresis in athlete’s urine and a pilot study of their changes responding to sport fatigue

    S. Ma, J. Ren and Z. Xu, Anal. Methods, 2019, Accepted Manuscript , DOI: 10.1039/C9AY00457B

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