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High-resolution colorimetric detection of lipase activity based on enzyme-controlled reshaping of gold nanorods

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Abstract

We developed a novel enzyme-controlling colorimetric assay to measure lipase activity. The method relied on enzymatic reaction-assisted gold deposition on gold nanorods (AuNRs) to generate a significant color change, which was strongly dependent on lipase activity. The H2O2 produced by autoxidation of the ethylene oxide subunits in Tween 80 reduced AuCl4 ions to Au atoms, which were coated onto of the surface of the AuNRs. In addition, lipase catalyzed the hydrolysis of the carboxyl ester bond in Tween 80, which controlled the rate of reshaping of AuNRs and tailored the localized surface plasmon resonance of the AuNRs. A linear response ranging from 0 to 4.5 mg mL−1 and a detection limit of 0.017 mg mL−1 for lipase were obtained. The methodology provided a sensitive, convenient, and rapid assay to monitor enzyme activities by judging different colors using both naked-eye detection and colorimetric instruments.

Graphical abstract: High-resolution colorimetric detection of lipase activity based on enzyme-controlled reshaping of gold nanorods

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Publication details

The article was received on 26 Feb 2019, accepted on 22 Mar 2019 and first published on 22 Mar 2019


Article type: Paper
DOI: 10.1039/C9AY00410F
Citation: Anal. Methods, 2019, Advance Article

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    High-resolution colorimetric detection of lipase activity based on enzyme-controlled reshaping of gold nanorods

    H. Zhang, S. Wu, L. Zhang, L. Jiang, F. Huo and D. Tian, Anal. Methods, 2019, Advance Article , DOI: 10.1039/C9AY00410F

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