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Automatic screening of antioxidants based on the evaluation of kinetics of a suppression of chemiluminescence in luminol – hydrogen peroxide system using sequential injection analysis set-up with the flow-batch detection cell

Abstract

Sequential injection analysis system with sensitive chemiluminescence detection using the flow-batch detection cell was developed for real time monitoring of kinetic profiles of antioxidant activity of phenolic substances in luminol-Fe(III)-hydrogen peroxide system during 60 s stop-flow period. Selected substances including coumaric acid, caffeic acid, hesperidin, ascorbic acid, resveratrol, rutin, chlorogenic acid, quercitrin, epicatechin, phloridzin, phloretin, and Trolox as a reference standard were tested with respect to different chemiluminescence signal kinetics. The evaluation of antioxidant activity was expressed as the quenching efficacy index Q. The method demonstrated wide working concentration range of 4 – 250 µmol L-1 for Trolox antioxidant activity. Additionally, the real time kinetic profiles based on chemiluminescence quenching half-life of both quick and slow antioxidants were distinguished. A method sample throughput of 30 h-1 was achieved. Antioxidant activity of apple and black elder fruit extracts was evaluated.

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Supplementary files

Publication details

The article was received on 21 Jan 2019, accepted on 09 Apr 2019 and first published on 10 Apr 2019


Article type: Paper
DOI: 10.1039/C9AY00160C
Citation: Anal. Methods, 2019, Accepted Manuscript

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    Automatic screening of antioxidants based on the evaluation of kinetics of a suppression of chemiluminescence in luminol – hydrogen peroxide system using sequential injection analysis set-up with the flow-batch detection cell

    G. Chládková, K. Kunovská, P. Chocholouš, M. Polášek and H. Sklenářová, Anal. Methods, 2019, Accepted Manuscript , DOI: 10.1039/C9AY00160C

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