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Issue 16, 2019
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Colorimetric assay of Hg2+ based on the inhibition of peroxidase mimetic activity of gold nanoclusters induced by Hg2+

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Abstract

In this paper, we report a highly sensitive and selective colorimetric assay for Hg2+ detection in aqueous solutions. Bovine serum albumin–gold nanoclusters (BSA–AuNCs) were prepared and used as a mimetic peroxidase to catalyze the 3,3′,5,5′-tetramethylbenzidine (TMB)–H2O2 system. In the absence of Hg2+, the AuNCs catalyze the reaction between TMB and H2O2, leading to a color change from colorless to blue. In the presence of Hg2+, owing to high-affinity metallophilic interactions between Hg2+ and Au+ on the surface of the AuNCs, Hg2+ can decrease the catalytic ability of the AuNCs, resulting in a decreased color change. The color changes in varying degrees depending on the concentration of Hg2+. The method shows low detection limits for Hg2+: 3.0 nM in deionized water and 3.5 nM in lake water.

Graphical abstract: Colorimetric assay of Hg2+ based on the inhibition of peroxidase mimetic activity of gold nanoclusters induced by Hg2+

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Publication details

The article was received on 15 Jan 2019, accepted on 16 Feb 2019 and first published on 18 Feb 2019


Article type: Paper
DOI: 10.1039/C9AY00109C
Citation: Anal. Methods, 2019,11, 2179-2182

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    Colorimetric assay of Hg2+ based on the inhibition of peroxidase mimetic activity of gold nanoclusters induced by Hg2+

    X. Jiang, W. Xu, X. Chen and Y. Liang, Anal. Methods, 2019, 11, 2179
    DOI: 10.1039/C9AY00109C

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