Jump to main content
Jump to site search


Development of a Whole-cell Biosensor for the Determination of Tyrosine in Urine for Point-of-care Diagnostics

Abstract

Most disorders associated with tyrosine metabolism lead to elevated tyrosine levels in biological fluids. In this work, we used genetic engineering techniques to develop a simple and low-cost biosensor for tyrosine quantification. Here, two tyrosine-responsive promoters are used to differentially control the production of red and green fluorescent proteins signals in response to tyrosine levels in culture. We also conducted a systematic examination of various modular components (promoters and ribosome binding sites) in order to ensure that our biosensor was capable of rapid producing pronounced fluorescence signal. To make the device more user-friendly, we developed a reagentless platform in which biocompatible agarose is used as an entrapment agent with cell sensors and growth media mounted within the gel matrix.

Back to tab navigation

Supplementary files

Publication details

The article was received on 10 Jan 2019, accepted on 04 Feb 2019 and first published on 05 Feb 2019


Article type: Paper
DOI: 10.1039/C9AY00070D
Citation: Anal. Methods, 2019, Accepted Manuscript

  •   Request permissions

    Development of a Whole-cell Biosensor for the Determination of Tyrosine in Urine for Point-of-care Diagnostics

    C. Lin, Q. Zhang and Y. Yeh, Anal. Methods, 2019, Accepted Manuscript , DOI: 10.1039/C9AY00070D

Search articles by author

Spotlight

Advertisements