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Issue 10, 2019
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Development of a whole-cell biosensor for the determination of tyrosine in urine for point-of-care diagnostics

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Abstract

Most disorders associated with tyrosine metabolism lead to elevated tyrosine levels in biological fluids. In this work, we used genetic engineering techniques to develop a simple and low-cost biosensor for tyrosine quantification. Here, two tyrosine-responsive promoters are used to differentially control the production of red and green fluorescent proteins signals in response to tyrosine levels in a culture. We also conducted a systematic examination of various modular components (promoters and ribosome binding sites) in order to ensure that our biosensor was capable of rapidly producing a pronounced fluorescence signal. To make the device more user-friendly, we developed a reagentless platform in which biocompatible agarose is used as an entrapment agent with cell sensors and growth media mounted within the gel matrix.

Graphical abstract: Development of a whole-cell biosensor for the determination of tyrosine in urine for point-of-care diagnostics

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Publication details

The article was received on 10 Jan 2019, accepted on 04 Feb 2019 and first published on 05 Feb 2019


Article type: Paper
DOI: 10.1039/C9AY00070D
Citation: Anal. Methods, 2019,11, 1400-1404

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    Development of a whole-cell biosensor for the determination of tyrosine in urine for point-of-care diagnostics

    C. Lin, Q. Zhang and Y. Yeh, Anal. Methods, 2019, 11, 1400
    DOI: 10.1039/C9AY00070D

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