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Issue 16, 2019
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An electrochemical immunosensor for prostate specific antigen using nitrogen-doped graphene as a sensing platform

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Abstract

In this study, a sensitive electrochemical immunosensor for prostate specific antigen (PSA) detection is described using nitrogen-doped graphene (NG) and gold nanoparticles (Au NPs) as a sensing interface. In this design, a PSA antibody (Ab) was first immobilized on the surface of NG decorated with Au NPs, and an aptamer labelled with ferrocene (Fc) was used as a signal probe. In the presence of PSA, a sandwich structure of Ab–PSA–aptamer was formed via specific recognition. Differential pulse voltammetry (DPV) was employed to record the electrochemical signal of Fc in phosphate-buffered saline (PBS) containing 0.10 M NaClO4. Under the optimal experimental conditions, the electrochemical immunosensor displayed a wide linear response range (0.05 ng mL−1 to 100 ng mL−1) and low detection limit (0.017 ng mL−1 (at S/N = 3)). Further, the immunosensor was evaluated with a serum sample, and satisfactory results were obtained.

Graphical abstract: An electrochemical immunosensor for prostate specific antigen using nitrogen-doped graphene as a sensing platform

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Publication details

The article was received on 09 Jan 2019, accepted on 19 Mar 2019 and first published on 21 Mar 2019


Article type: Paper
DOI: 10.1039/C9AY00064J
Citation: Anal. Methods, 2019,11, 2183-2189

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    An electrochemical immunosensor for prostate specific antigen using nitrogen-doped graphene as a sensing platform

    W. Meng, W. Zhang, J. Zhang, X. Chen and Y. Zhang, Anal. Methods, 2019, 11, 2183
    DOI: 10.1039/C9AY00064J

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