Fabrication of an eco-friendly ratiometric fluorescence sensor modified mesoporous structured epitope imprinted polymer for highly selective and sensitive determination of cytochrome c in biological samples
A facile and environmental friendly strategy was applied to prepare ratiometric fluorescence sensor modified mesoporous structured epitope imprinted polymer (QDs@SiO2@EMSiO2) for highly sensitive and selective determination of cytochrome c (Cyt c) using Cu-doped ZnS QDs and Mn-doped ZnS QDs as fluorescence dyes. In this sensor, C-terminate and N-terminate nanopeptides were employed as the template molecule and C18MIMCl was utilized as template for mesoporous silica. APTES and PTESMIC were utilized as the functional monomer and TEOS was applied as the cross-linker. The as-prepared fluorescence sensor displayed great selectivity and sensitivity by merging ratiometric fluorescence method with mesoporous silica materials and creating one system. The composite was employed for the recognition of Cyt c and displayed a good linearity between 0.4 to 200.0 nM and with the detection limit as low as 0.15 nM which was much lower than those non-mesoporous and single emission one. The sensor also illustrates outstanding applicability in complex matrices which enables it to employ in Cyt c tracing in human serum samples without any pretreatments.