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A novel highly selective near-infrared and naked-eye fluorescence probe for imaging peroxynitrite

Abstract

Peroxynitrite (ONOO−) is closely related to various physiological and pathological processes. Real-time, selective, and sensitive detection of ONOO− is of great importance. Based on the intramolecular charge transfer (ICT) mechanism, a novel near-infrared (NIR) and naked-eye fluorescence probe DCIPP was developed with “turn-on” fluorescence property for monitoring ONOO− in living cells. The probe DCIPP is composed of a dicyanoisophorone electron-accepting scaffold and a reactive electron-drawing diphenyl phosphoryl group for imaging ONOO–. Upon addition of ONOO–, the fluorescence intensity of DCIPP enhances obviously at 665 nm in emission spectra, and the colour of DCIPP solution changes from pale yellow to purple at the same time. Based on the nucleophilic propensity of ONOO–, this probe displays high selective detection of ONOO– over other various bioactive molecules and could quantitatively and sensitively detect ONOO– with a low limit of detection (LOD) of 33 nM by fluorescence method and 0.29 μM by UV method. Moreover, DCIPP was successfully applied to the fluorescence imaging of ONOO− in living cells and zebrafish with good cell-membrane permeability and low cytotoxicity.

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Publication details

The article was received on 02 Dec 2018, accepted on 06 Feb 2019 and first published on 12 Feb 2019


Article type: Paper
DOI: 10.1039/C8AY02632G
Citation: Anal. Methods, 2019, Accepted Manuscript

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    A novel highly selective near-infrared and naked-eye fluorescence probe for imaging peroxynitrite

    R. Yuan, Y. Ma, J. Du, F. Meng, J. Guo, M. Hong, Q. Yue, X. Li and C. Li, Anal. Methods, 2019, Accepted Manuscript , DOI: 10.1039/C8AY02632G

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