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A sensitive and regenerative electrochemical immunosensor for quantitative detection of Escherichia coli O157:H7 based on stable polyaniline coated screen-printed carbon electrode and rGO-NR-Au@Pt

Abstract

A sensitive and quantitative sandwich electrochemical immunosensor was constructed for detection of Escherichia coli O157:H7 (E. coli O157:H7) using immune bimetallic nanoparticles Au@Pt, neutral red, reduced graphene oxide nanocomposite (rGO-NR-Au@Pt) and reusable leucoemeraldine base polyaniline/gold nanoparticles modified screen-printed carbon electrode (SPCE-PANI-AuNPs). The advantages of batch production of SPCE were retained, the characteristics of its disposable use was replaced by the possibility of reuse. The anti-E. coli O157:H7 monoclonal antibody was automatic adsorbed on SPCE-PANI-AuNPs platform through amino and AuNPs interaction. rGO-NR-Au@Pt as the non-enzyme signal label can enhance the performance of the immunoassay for the catalytic reduction of H2O2 and amplify the current signal. All characterization steps were monitored by cyclic voltammetry (CV). Based on electrochemical detection of E. coli O157:H7, linear range was from 8.9 ×103 to 8.9 ×109 CFU/mL, and limit of detection (LOD) was 2.84 ×103 CFU/mL. To ensure that the immunosensor can be used repeatedly, ultrasound sonication is used to remove as much surface-bound antibody as possible. Therefore, the antibody can bind to the SPCE-PANI-AuNPs again after the electrode is re-incubated with the antibody solution, and hence, the displacement assay can be performed again for repeated sensor use. Furthermore, the microchips can be reusable for at least up to 5 times as demonstrated herein.

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Publication details

The article was received on 28 Nov 2018, accepted on 10 Jan 2019 and first published on 12 Jan 2019


Article type: Paper
DOI: 10.1039/C8AY02594K
Citation: Anal. Methods, 2019, Accepted Manuscript
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    A sensitive and regenerative electrochemical immunosensor for quantitative detection of Escherichia coli O157:H7 based on stable polyaniline coated screen-printed carbon electrode and rGO-NR-Au@Pt

    X. Mo, Z. Wu, J. Huang, G. Zhao and W. Dou, Anal. Methods, 2019, Accepted Manuscript , DOI: 10.1039/C8AY02594K

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