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Determination of Urea Concentration Using Urease-containing Polyelectrolyte Microcapsules.

Abstract

Nowadays, enzymological method in clinical diagnostics has several problems: a short lifetime of the enzyme-sensor in solution, its defenselessness in the presence of proteolytic enzymes, and the impossibility of multiple uses. We have proposed the modification of the standard method of urea detection. This modification will solve the problems posed above. The main idea of the proposed approach is encapsulation of urease into microcapsules obtained by the method of alternate adsorption of oppositely charged polyelectrolytes. The determination was made with the help of unfixed (microdiagnosticum) and fixed microcapsules on a solid surface (diagnostic plate). Multiple uses of microdiagnosticum and diagnostic plate has been shown, the results obtained have deviations and do not exceed the value of 9.6%. We determined the urea concentration in the serum blood by the standard Berthelot method using a diagnostic plate and using a microdiagnosticum for detection in the urine. The results obtained differing from results obtained with free enzyme by no more than 4.26% for the diagnostic plate and 1.67% for microdiagnosticum. The diagnostic plate has a range of detectable concentrations of 5-50 mmol / L, linearity up to 40 mmol / L, deviation from linearity does not exceed 10%, while the coefficient of variation does not exceed 7.17. Microdiagnosticum has a range of detectable concentrations of 5-55 mmol / L, linearity up to 50 mmol / L, deviation from linearity does not exceed 7%, the coefficient of variation does not exceed 2.85.

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Publication details

The article was received on 13 Nov 2018, accepted on 25 Jan 2019 and first published on 04 Feb 2019


Article type: Paper
DOI: 10.1039/C8AY02481B
Citation: Anal. Methods, 2019, Accepted Manuscript

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    Determination of Urea Concentration Using Urease-containing Polyelectrolyte Microcapsules.

    A. Kim, E. Musin, A. Dubrovskii and S. Tikhonenko, Anal. Methods, 2019, Accepted Manuscript , DOI: 10.1039/C8AY02481B

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