Jump to main content
Jump to site search


Highly Sensitive Optical Biosensing of Staphylococcus aureus with an Antibody/Metal-Organic Framework Bioconjugate

Abstract

In this research, a new luminescent bioprobe was developed for the detection of S. aureus based on bio-conjugation of an amine functionalized metal-organic framework (NH2-MIL-53(Fe)) with an anti-S. aureus antibody (Ab). The formation of the desired bioprobe (Ab/NH2-MIL-53), in its liquid phase, has been verified with several spectroscopic and structural characterizations. The bioprobe was incubated with varying concentrations of S. aureus bacteria. The resulting antibody conjugated bioprobe (Ab/NH2-MIL-53) maintained a strong inverse correlation in which decreases in fluorescence intensity were accompanied by increase in bacterial count. Thus, the potential of the herein developed probe has been successfully demonstrated for the detection of S. aureus with a low limit of detection (85 CFU/mL) over a wide concentration range (40 × 102 - 4 × 108 CFU/mL). It was further found to be reliable with regard to inter-/intra- precision assays and long-term stability. The feasibility of the method was further supported through the detection of S. aureus spiked in environmental samples (e.g., river water and cream pastry).

Back to tab navigation

Publication details

The article was received on 13 Nov 2018, accepted on 05 Jan 2019 and first published on 07 Jan 2019


Article type: Paper
DOI: 10.1039/C8AY02476F
Citation: Anal. Methods, 2019, Accepted Manuscript
  •   Request permissions

    Highly Sensitive Optical Biosensing of Staphylococcus aureus with an Antibody/Metal-Organic Framework Bioconjugate

    N. Bhardwaj, S. Kumar, D. Bhatt, S. K. Tuteja, K. Kim and A. Deep, Anal. Methods, 2019, Accepted Manuscript , DOI: 10.1039/C8AY02476F

Search articles by author

Spotlight

Advertisements