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Issue 2, 2019
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Duplex-specific nuclease-mediated target recycling amplification for fluorescence detection of microRNA

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Abstract

Isothermal amplification techniques for nucleic acid detection have drawn increasing interest recently due to the simplicity and low-cost of instruments. We have developed a novel duplex-specific nuclease (DSN)-mediated target recycling amplification strategy for microRNA detection. The method utilizes the different adsorption ability of graphene oxide (GO) between single-stranded DNA and double-strand DNA to achieve fluorescence recovery. DSN was employed to mediate target recycling amplification, and desirable sensitivity for miR-21 detection was achieved. The developed method also demonstrated excellent selectivity and applicability for analysis of samples in complex biological media. The application of the developed method for analysis of miR-21 level in three different cell lines demonstrated its potential for real sample analysis.

Graphical abstract: Duplex-specific nuclease-mediated target recycling amplification for fluorescence detection of microRNA

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Publication details

The article was received on 17 Oct 2018, accepted on 26 Nov 2018 and first published on 30 Nov 2018


Article type: Paper
DOI: 10.1039/C8AY02265H
Citation: Anal. Methods, 2019,11, 200-204

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    Duplex-specific nuclease-mediated target recycling amplification for fluorescence detection of microRNA

    L. Tan, L. Xu, J. Liu, L. Tang, H. Tang and R. Yu, Anal. Methods, 2019, 11, 200
    DOI: 10.1039/C8AY02265H

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