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Issue 3, 2019
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Whole-cell biosensing by siderophore-based molecular recognition and localized surface plasmon resonance

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Abstract

A siderophore-based active bacterial pull-down strategy was integrated in a localized surface plasmon resonance (LSPR) sensing platform and subsequently tested by detecting whole-cell Acinetobacter baumannii. The LSPR-based whole-cell sensing approach was previously demonstrated with aptamer-based molecular recognition motifs, and here it is extended to the powerful siderophore system, which exploits the natural bacterial need to sequester Fe(III). Specifically, a biscatecholate–monohydroxamate mixed ligand siderophore linked to a biotin via three polyethylene glycol repeating units was synthesized and immobilized on Au trigonal nanoprisms of an LSPR sensor. The resulting surface-confined biotinylated siderophore subsequently chelated Fe(III), forming a siderophore–Fe(III) complex which was shown to be competent to recognize A. baumannii. Target bacteria were captured and then detected by measuring wavelength shifts in the LSPR extinction spectrum. This siderophore pull-down LSPR biosensor approach is rapid (≤3 h detection) and sensitive – with a limit of detection (LOD) of 80 bacterial cells and a linear wavelength shift over the range 4 × 102 to 4 × 106 cfu mL−1. As intended by design, the siderophore-based biosensor was selective for A. baumannii over Pseudomonas aeruginosa, Escherichia coli, and Bacillus cereus, and was stable in ambient conditions for up to 2 weeks.

Graphical abstract: Whole-cell biosensing by siderophore-based molecular recognition and localized surface plasmon resonance

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Publication details

The article was received on 05 Oct 2018, accepted on 05 Dec 2018 and first published on 17 Dec 2018


Article type: Paper
DOI: 10.1039/C8AY02180E
Citation: Anal. Methods, 2019,11, 296-302
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    Whole-cell biosensing by siderophore-based molecular recognition and localized surface plasmon resonance

    J. Hu, M. Ghosh, M. J. Miller and P. W. Bohn, Anal. Methods, 2019, 11, 296
    DOI: 10.1039/C8AY02180E

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