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A highly sensitive and selective fluorescent probe for nitroxyl based on a naphthalene derivative

Abstract

In this work, we developed a novel fluorescent probe 1 for quantitative detection of nitroxyl (HNO). Probe 1 consisted of a naphthalene backbone and 2-(diphenylphosphino)benzoate which was used as a nitroxyl recognition unit. The probe exhibited an intense fluorescence turn-on response to nitroxyl via the aza-ylide formation and its subsequent Stadinger ligation to release compound 2. The probe can be applied to the quantification of nitroxyl with a linear range covering from 5.0×10-8 to 9.0×10-6 mol•L-1. The detecting limit of probe 1 toward nitroxyl was estimated to be 43 nM. Furthermore, probe 1 displayed a much higher selectivity for nitroxyl than other biologically relevant species. Importantly, owing to the high cell permeability and low cytotoxicity of probe 1, it was successfully applied to cell imaging of nitroxyl in living cells. The probe was expected to be a useful chemical tool for investigating the detailed functions and mechanisms of HNO in living systems.

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Publication details

The article was received on 15 Sep 2018, accepted on 04 Jan 2019 and first published on 07 Jan 2019


Article type: Paper
DOI: 10.1039/C8AY02030B
Citation: Anal. Methods, 2019, Accepted Manuscript
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    A highly sensitive and selective fluorescent probe for nitroxyl based on a naphthalene derivative

    Q. Ma, J. Xu, G. Mao, X. Guo, B. Liang, Y. Bai and C. Wang, Anal. Methods, 2019, Accepted Manuscript , DOI: 10.1039/C8AY02030B

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