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A target-driven DNA-based molecular machine for rapid and homogeneous detection of arginine-vasopressin

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Abstract

Rapid detection of physiological changes of neuropeptides is of great importance as they are involved in a wide range of physiological processes and behaviors. Abnormalities in their expression level are correlated with various neurological diseases. However, current methods such as radioimmunoassay, enzyme-linked immunosorbent assays and liquid chromatography tandem mass spectrometry relied on cumbersome operation steps and could not rapidly provide the information of their concentration fluctuations. Thus motivated, we developed a target-driven DNA-based molecular machine that could be triggered only in the presence of a specific target neuropeptide. Using arginine-vasopressin (AVP) as a model neuropeptide, we integrated the DNA-based molecular machine with fluorescence signal transduction and amplification technology. The assay was rapid and homogeneous, which offered a linear range of 75–700 pM and a limit-of-detection as low as 75 pM. It holds great potential for further applications in real-time monitoring of the variations of the AVP level in biological samples.

Graphical abstract: A target-driven DNA-based molecular machine for rapid and homogeneous detection of arginine-vasopressin

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Publication details

The article was received on 15 Oct 2019, accepted on 19 Nov 2019 and first published on 21 Nov 2019


Article type: Paper
DOI: 10.1039/C9AN02060H
Analyst, 2020, Advance Article

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    A target-driven DNA-based molecular machine for rapid and homogeneous detection of arginine-vasopressin

    H. Tan, L. Chen, X. Li, M. Li and M. Zhao, Analyst, 2020, Advance Article , DOI: 10.1039/C9AN02060H

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